AFP and Ascites: For plasma samples, the results showed a significant association with high level of ALT (P. value <0.001), low albumin (P. value =0.01), high level of AFP (P. value <0.01), higher number of masses (P. value <0.001), larger tumor size (P. value <0.001), advanced tumor grade III (P. value <0.001), Acinar/Solid pattern (P. value <0.001), HAI A3 (P. value <0.001), Hepatomegaly (P. value <0.001), Ascites (P. value <0.001), Splenomegaly (P. value <0.001), and in patients with lower limb edema (P. value <0.001).