Three (2m/1f) interleukin-10-deficient (Il10-/-) mice (129Sv/Ev background) were reared germ-free to adulthood (8–10 weeks) and colonzied by oral gavage with an even mixture of a total of 107 CFU clinical E. coli strains isolated from the intestinal tissue of Crohn’s disease and non-Crohn’s disease patients [7]. Here, IL10 is linked to Crohn disease.