ASB16-AS1 expression were positively correlated with TMB in 4 cancer types: ACC (r = 0.36, p =0.001), UCEC (r =0.28, p <0.001), LGG (r =0.24, p <0.001), BLCA (r =0.14, p =0.004), and negatively correlated with 3 cancer types: THYM (r = −0.20, p =0.033), BRCA (r =−0.13, p <0.001), LGG (r =−0.10, p =0.040) (Figure 6b). The gene discussed is ASB16; the disease is cancer.