Our results showed that the levels of NCF expression are significantly downregulated in multiple cancer types including LUAD (lung adenocarcinoma) and LUSC (lung squamous cell carcinoma), but were higher in GBM (glioblastoma multiforme), KIRC (kidney renal clear cell carcinoma), and KIRP (kidney renal papillary cell carcinoma). This evidence concerns the gene NCF4 and squamous cell lung carcinoma.