ROC-based analysis results revealed that Apelin<2.225 ng/mL at T1 had a sensitivity of 58.33% and a specificity of 70.05% in forecasting HT in CIS patients after IT (all P < 0.001), and Vaspin <1.085 ng/mL at T1 had a sensitivity of 72.92% and a specificity of 72.13% in forecasting it (all P < 0.001). This evidence concerns the gene APLN and in situ carcinoma.