In both the CD8+ and CD4+ TIL subsets, we observed that the expression of genes in the activation gene sets increased as reactivity marker (CD137, TNF, or IFNγ) gene positivity increased, i.e., the highest expression values for the majority of tumor-specific activation genes were observed in the Triple Positive cluster (TNFRSF9+TNF+IFNG+). This evidence concerns the gene CD4 and neoplasm.