Based on our data, and on previous studies (10, 30) showing BFA to be optimal for detecting TNF and IFNγ, and MN to be optimal for detecting CD107a, the combination of BFA and MN was chosen to maximize the detection of tumor-specific reactive TILs on a protein level by intracellular staining after exposure to autologous TCLs. Here, LAMP1 is linked to neoplasm.