In addition, pAkt/Akt was also increased in both groups following the treatment with TGFβ1 1 ng/ml with insulin (healthy: P < 0.01, PCOS: P < 0.001, Figure 3B) and TGFβ1 5 ng/ml with insulin (healthy: P < 0.05, PCOS: P < 0.001, Figure 3B). This evidence concerns the gene INS and polycystic ovary syndrome.