Then, murine CRC CT26 cells with Mysm1 knocked out via CRISPR-Cas9 (Additional file 12: Figure S2A and B) and SW620 cells infected with a lentivirus to construct stable MYSM1-overexpressing cell lines (Fig. 3A) were subcutaneously injected into athymic BALB/c mice. The gene discussed is MYSM1; the disease is colorectal carcinoma.