The results showed that the expression of NEK2 in the HepG2 and Hep3B HCC cells was significantly higher than that in the normal liver HL7702 cells, and the protein was mainly located in the cytoplasm, which was consistent with the immunohistochemical results of our previous experiment using a mouse anti-human NEK2 monoclonal antibody (Abcam, USA) [9]. The gene discussed is NEK2; the disease is hepatocellular carcinoma.