To explore the effect of the anti-NEK2 mAb specifically binding corresponding antigens on the growth and proliferation of HCC cells, we used HepG2 and Hep3B cells with high expression of NEK2 protein in this study, and the prepared anti-NEK2 3A3 mAb was co-cultured with HCC cells to observe the effect of antibodies on HCC cells. Here, NEK2 is linked to hepatocellular carcinoma.