CKB and cancer: To test our hypothesis that CKB downregulation promotes cancer progression via AKT S473 phosphorylation, we first confirmed that CKB stable silencing by shRNA and CRISPR/Cas9/gRNA increases, while its cDNA ectopic expression decreases, p-S473-AKT levels in PC3 and DU145 cells (Fig. 5B−C).