In LIRI+parecoxib group, 10mg/kg parecoxib was given via femoral vein 15 min before ischemia beginning.At the end of the reperfusion, blood gas analysis, lung wet to dry massratio measurement, lung tissue biochemical determination and hemeoxygenase-1 (HO-1) protein expression determination were performed. This evidence concerns the gene HMOX1 and ischemia.