The present study aimed to analyze the induction of antigen-specific tolerance after prophylactic immunization with a DNA vaccine encoding the epitopes: D183-119, D2, B ́/B, and B ́/BCOOH in co-vaccination with IFN-γ or IL-10 in a murine model of lupus induced by pristane. The gene discussed is IL10; the disease is systemic lupus erythematosus.