Another early example of PrOF NMR was described by the Norton lab focusing on the SPRY domain-containing SOCS box protein 2 (SPSB2), a target implicated in infectious diseases for its role in recruiting an E3 ligase to inducible nitrous oxide synthase (iNOS) for degradation.119 In this case, PrOF NMR was used as a secondary validation assay. This evidence concerns the gene SPSB2 and infectious disease.