Results of flow cytometry analysis showed that AOM/DSS treatment upregulated the percentage of CD25+FoxP3+ T cell (Treg) in IEL separated from both Tipe2-/- mice and Tipe2+/+ (Fig. 7A), but was significantly lower in AOM/DSS-induced Tipe2-/- mice (Fig. 7A). This evidence concerns the gene TIPE2 and infectious otitis media.