Similarly to T-REx 293 cells, in A375 (Figure 4H), A375 IV (Figure 4I), and A2058 (Figure 4J and quantified effect of RNF43 OE on the endogenous WNT pathway activity in Figure 4—figure supplement 1G and H) melanoma cells RNF43 overexpression efficiently blocked WNT5A-induced signaling. Here, WNT5A is linked to melanoma.