To detect the correlation between E2F1 and miR-375 in patients, miR-375 and E2F1 expression in ESCC tissues of 38 patients were listed and subjected to Person’s correlation analysis, with confidence intervals chosen as 95% and R-values as curve fit, showing a significant negative correlation between miR-375 and E2F1 (Figure 4e). This evidence concerns the gene E2F1 and esophageal squamous cell carcinoma.