So p-IκBα and p65 expression was detected by Western blot, and the data showed that miR-4521 overexpression remarkably restrained p-IκBα and p65 expression in BC cells, while the restoration of HURP promoted the expression of p-IκBα and p65 (P < 0.01) (Figure 6b); inhibition of miR-4521 induced p-IκBα and p65 expression in BC cells, and knockdown of HURP exerted the opposite effect (P < 0.01) (Figure 6b). Here, NFKBIA is linked to breast cancer.