PITX2 and neoplasm: For that purpose we selected four targets (TMEFF2 (p < 10−7), PITX2 (p < 10−7), TWIST1 (p < 10−7), TFPI2 (p < 10 × 2.3−6)) with highly significant differential methylation; DCC with moderate significant differential methylation (p= 0.014) and PTGS2 lacking differential methylation (p = 0.29) between the tumour group and the adjacent tissue group.