THRAP3 and cancer: Using a proximity ligation assay with the S9.6 antibody, which can detect R-loops, and an anti-Thrap3 antibody, we detected the colocalization of Thrap3 with R-loops in the nuclear region; in addition, depletion of Thrap3 using siRNA decreased the ligation signals in both cancer and normal (MEF) cells (Fig. 1a, Supplementary Fig. 1a, b).