We demonstrated that the upregulation of TMEM44-AS1 activated by Myc and the effect of TMEM44-AS1 on downstream targets Myc and EGR1/IL-6 is dependent on SerpinB3, and the interaction of Myc with MED1 bound to the TMEM44-AS1 SE and promoted its glioma-specific transcription activation in glioma progression. Here, EGR1 is linked to glioma.