To evaluate the biofunctional role of TMEM44-AS1 in glioma cells, we interfered with endogenous TMEM44-AS1 expression by specific siRNA or lentivirus-expressing shRNA specific to TMEM44-AS1, or TMEM44-AS1-overexpressing plasmid, and then we confirmed the transfection efficiency by RT-qPCR analysis (FigS.1A-C). Here, TMEM44 is linked to central nervous system cancer.