We then analyzed the relationship between hsa-mir-7-5p, hsa-mir-145-5p, SOX21-AS1, mTOR and KLF4 co-expression (more than 260 sample sizes) by transcriptome sequencing results of osteosarcoma from the database, and further prove the above conclusion (Figure 3(h-j)). This evidence concerns the gene KLF4 and osteosarcoma.