In summary, we hypothesize that lncRNA SOX21-AS1 may enhance the proliferative properties of osteosarcoma by upregulating mTOR and KLF4, and verify whether ginsenoside Rg3 can inhibit osteosarcoma proliferation by targeting lncRNA SOX21-AS1, thus hopefully providing an alternative for future clinical treatment of osteosarcoma. This evidence concerns the gene KLF4 and osteosarcoma.