To confirm the regulation of miR-326 targeting SIRT1 in chemotherapy resistance of NSCLC cells, chemotherapy resistant H460-R cells were co-transfected with NC mimic and oe-NC, miR-326 mimic and oe-NC, or miR-326 mimic and oe-SIRT1. This evidence concerns the gene SIRT1 and non-small cell lung carcinoma.