The presence of ZIKVBR in the 3D brain organoid, with or without tumor cells, upregulated the B2M transcript, that displays anti-pathogenic activity in amniotic fluid, and downregulated the cytokine Spp1, associated tumorigenesis, and the growth factor Nodal, required for the maintenance of human embryonic stem cell pluripotency (Figure 5F). Here, NODAL is linked to neoplasm.