SPP1 and neoplasm: The presence of ZIKVBR in the 3D brain organoid, with or without tumor cells, upregulated the B2M transcript, that displays anti-pathogenic activity in amniotic fluid, and downregulated the cytokine Spp1, associated tumorigenesis, and the growth factor Nodal, required for the maintenance of human embryonic stem cell pluripotency (Figure 5F).