VCP and infection: To investigate whether VCP ATPase is required for DENV replication, Huh7.5 hepatocarcinoma cells were infected with the DENV (serotype 2, strain 16681s) at a multiplicity of infection (MOI) of 0.05, and treated for 24 h with 2 selective VCP ATPase inhibitors, namely CB-5083 or NMS-873, which exhibit 2 different modes of action (ATP-competitive vs. allosteric non-ATP-competitive, respectively) [47,48,49,50].