To determine the pattern of MAVS ubiquitination in aMPV/C-infected Vero cells, we co-transfected Vero cells with plasmids encoding Flag-MAVS or Flag and HA-Ub-K48 or HA-Ub-K63, followed by aMPV/C infection and an immunoprecipitation assay. Here, MAVS is linked to infection.