To elucidate whether PD-H spreads to distant Colon-26 tumors, we established two subcutaneous Colon-26 tumors on both flanks of Balb/C mice, injected one tumor with 1 × 106 pfu of PD-H and investigated the animals 2, 3 and 7 days later. This evidence concerns the gene PDP1 and neoplasm.