MMUT and neoplasm: These two minigenes were then transfected individually into tumor cell lines KYSE140 (HLA-A2+) and KYSE150 (HLA-A2−) to obtain KYSE140-WT (HLA-A2+, MUT peptide−), KYSE140-MUT (HLA-A2+, MUT peptide+), KYSE150-WT (HLA-A2−, MUT peptide−), and KYSE150-MUT (HLA-A2−, MUT peptide+) target cells.