PCLO and neoplasm: To figure out whether the MUT peptides TP53-R267P, NFE2L2-D13N, and PCLO-E4090Q were endogenously processed and presented by transfected tumor cells, and whether MUT peptide-specific CTLs could recognize these MUT peptides presented on the surface of transfected tumor cells, we constructed two minigenes (containing WT or MUT sequences) that encoded these three MUT peptides or their corresponding WT peptides (described in Supplemental Materials).