The AIOLOS N160S mutation stood out as the best candidate variant, because multiple sequence alignments of IKAROS and AIOLOS protein sequences implied that AIOLOS N160 is homologous to IKAROS N159, the site of an IKAROS variant (N159S) that previously has been associated with CID and PJP (Fig. 1 b; Boutboul et al., 2018). The gene discussed is IKZF1; the disease is combined immunodeficiency.