To further confirm miR-101-3p regulated cisplatin sensitivity by regulation of ATG4D and autophagy, we co-transfected with miR-101-3p inhibitor and ATG4D siRNA to NSCLC cells, and compared the changes of cell activity, autophagy-related protein expression, number of autophagosomes and autolysosomes, and autophagy ultrastructure by CCK-8 assay, Western blot, confocal microscopy and TEM analysis, respectively. This evidence concerns the gene ATG4D and non-small cell lung carcinoma.