To this end, we subjected mice to a similar challenge and treatment regime as described above and included an additional study arm of microbiota depleted animals (Fig S1c), known to precipitate similar DSS-induced intestinal injury as conventional mice.51 Moreover, the experiment was performed in a new vivarium and with mice from a different vendor in order to ensure reproducibility of lysozyme-mediated protection against DSS-induced colitis (DSS-induced colitis is notoriously difficult to phenocopy across cohorts52). Here, LYZ is linked to colitis.