L1CAM and neoplasm: In a western blot analysis, we were able to confirm a different regulation of L1-CAM and CD44 in both clones compared to the respective control samples using protein lysates obtained from tumours of the resected mice (Fig. 2a) However, we could only detect a higher expression of ICAM-1 in Fra-2 cl 2 (Fig. 2a), but by immunohistochemical staining of sections from primary tumours and metastases of lung tissue, we could validate expression differences of ICAM-1 between both Fra-2 clones compared to the control (Fig. 3b).