Four weeks after transplantation, FACS analysis showed that the mice transplanted with wild-type leukemic cells had higher GFP+ leukemic cell populations in the peripheral blood compared to the mice received with Ash1L-KO leukemic cells (Figure 3B), which was consistent with the higher leukemic cell numbers in the peripheral blood smears and splenomegaly found in the mice transplanted with wild-type leukemic cells (Figures 3C, D). This evidence concerns the gene ASH1L and Splenomegaly.