In order to explore whether HPV E6 takes part in the carcinogenic progress of cervical cancer by regulating G6PD, the G6PD expressional upregulation (plenti G6PD) or downregulation (G6PD siRNA) were conducted in HeLa cells on the basis of the HPV16 E6 siRNA and/or overexpressing, respectively. The gene discussed is G6PD; the disease is cervical cancer.