In Chinese Bama miniature pigs, zygote co-injection of Cas9 mRNA and sgRNA (single guide RNA) was used to delete Npc1l1 (Niemann-Pick C1-Like 1), efficiently producing biallelic mutant pigs to study how Npc1l1 influences cardiovascular and metabolic diseases [51]. Here, NPC1L1 is linked to metabolic disease.