Multivariate Cox regression analysis was conducted to develop a novel signature using fifteen cancer stemness-related genes (including RAB31, COL6A3, COL5A2, CCDC80, ADAM12, VGLL3, ECM2, POSTN, DPYSL3, PCDH7, CRISPLD2, COLEC12, NRP2, ISLR, and CCDC8) for prognosis prediction of COAD using the train cohort. This evidence concerns the gene ECM2 and colon adenocarcinoma.