To clarify the prognostic significance of TNF family, we conducted univariate Cox regression of all 46 proteins, and found that 17 proteins were significantly correlated with overall survival of HNSCC patients, of which only TNFRSF12A, EDA, LTBR and EDA2R predicted unfavorable outcomes (Figure 2B). This evidence concerns the gene EDA and head and neck squamous cell carcinoma.