To evaluate the expression and localization of TGF-β1 in CCI model, we analyzed ipsilateral dorsal horns cell population (i.e., NeuN positive neurons, GFAP positive astrocytes, and Iba1 positive microglial cells) and their potential colocalization or proximity to TGF-β1 in the early phase of the neuropathy (Figures 5A–C). The gene discussed is GFAP; the disease is neuropathy.