As shown in Figure 5D, the major 10 KEGG pathways were significantly associated with various physiological processes, including NOD-like receptor signaling pathway, Toxoplasmosis, TNF signaling pathway, Proteoglycans in cancer, Ras signaling pathway, NF-kappa B signaling pathway and PI3K-Akt signaling pathway. The gene discussed is TNF; the disease is toxoplasmosis.