VIM and carcinoma: On the other hand, the metastasis related genes were examined by IHC assays, and it was found that E-cadherin was up-regulated; Vimentin and MMP9 were down-regulated in GA-treated tumoral or stromal tissue (Figures 7C,D), suggesting that GA inhibited the EMT polarization and blocked the potential of metastatic dissemination of primary carcinoma cells in vivo.