To explore whether KTN1 may specifically regulate EGFR protein levels in tumor cell lines, we transfected siKTN1 and siKTN1# into the human immortalized keratinocytes cell line HaCaT, in addition, KTN1mut was also transfected into the cell line to demonstrate the gene-specific of siKTN1. The gene discussed is KTN1; the disease is neoplasm.