Quantitative PCR (Fig. 3B, D) and western blotting (Fig. 3C, E) profiling indicated that HES5, but neither HEY1 nor HEY2, was downregulated in the livers of the NAFLD mice compared to the control mice in the MCD model and the HFD model. The gene discussed is HEY2; the disease is metabolic dysfunction-associated steatotic liver disease.