TPT1 and ischemic cardiomyopathy: To evaluate the role of fortilin in the heart and in human HF, we subjected tissue lysates of human hearts from subjects with non-failing hearts (NFHs) and HF patients with non-ischemic cardiomyopathy (NICM) and ischemic cardiomyopathy (ICM) from the Duke Human Heart Repository [18] to an automated capillary-based quantitative Western blot analyses (JESSTM, Protein Simple) [19, 20] (Fig. 1a).