ZSWIM8 and osteosarcoma: It was not known whether ZSWIM8 is expressed ubiquitously, and therefore, we first examined several available cell lines, such as HEK293T (human embryonic kidney cell), 786-O (human renal cell adenocarcinoma), K562 (human chronic myelogenous leukemia), MCF10A (human fibrocystic disease), RCC4 (human renal clear cell carcinoma), U-2 OS (human osteosarcoma), and C2C12 (mouse myoblast) (Fig. 1A).