Thus, to investigate the functional roles of JWA in trastuzumab-resistant breast cancer cells, we established stable cell lines (JWA) via lentiviral infection in JIMT1 and BT474-Tr cells and knocked down endogenous JWA via two independent siRNAs in the JIMT1 and BT474-Tr cells (Fig. 2C−F). Here, ARL6IP5 is linked to infection.