Figure 1A showed that after hM-CSF and 50% osteosarcoma-conditioned medium treatment (P < 0.01), the expression levels of macrophage-associated molecules including CD68, CD163, CD204, IL-10, and CCL1 in CD14+ PBMCs were significantly increased, which showed that TAMs-like macrophages were successfully induced. The gene discussed is CD163; the disease is osteosarcoma.