Interestingly, in neurofunctional tests after day-60 of differentiation, both SOD1 ALS MNs (SOD1G85R and SOD1D90A, but not SOD1G85G and SOD1D90D) triggered 488-nm fluorescence signals, which were followed by cell death immediately after glutamate treatment. Here, SOD1 is linked to amyotrophic lateral sclerosis.