When liver fibrosis was induced with carbon tetrachloride in wild-type, Nrf2−/−, p62−/− and Nrf2−/−p62−/− double-KO mice, the protective effect of SPD was significantly reduced in Nrf2 and p62 single-KO mice and was completely lost in double-KO mice [57]. This evidence concerns the gene SQSTM1 and Hepatic fibrosis.