SIRT6 has also been shown to affect TGF-β in HEK293 cells by deacetylating lysine 54 of mothers against decapentaplegic homolog (SMAD) 2- profibrogenic transcription factor, inhibiting its cytosol to nucleus translocation induced by TGF-β and its recruitment to the promoters of fibrogenesis genes; Col1a1 and Col1a2 acting protectively against liver fibrosis [27]. This evidence concerns the gene SIRT6 and Hepatic fibrosis.