Although migrations of breast cancer cells expressing the S108A mutation are normal in a 2D migration assay, they show diminished ability to cross a matrigel layer in a 3D transwell migration assay commonly used to measure invasive potential, suggesting O-GlcNAcylated cofilin has some specialized function in invadopodium formation or delivery/secretion of matrix metalloproteases. The gene discussed is CFL1; the disease is breast carcinoma.