The synthetic model foresees the presence of six key clones, hereafter named after single genes harboring specific variants, including four nested ones originating in PT, three persisting in all tumor samples and characterized by variants in CDKN2A, ALDH18A1, and TGFBR3, respectively, while the fourth with a change in ALDH1B1 was not detected in REC-3D (Figure 2e). This evidence concerns the gene TGFBR3 and neoplasm.